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Journal: Clinical & Translational Immunology
Article Title: Assessment of antibodies in the upper and lower human respiratory tract at steady state and after respiratory viral infection
doi: 10.1002/cti2.1460
Figure Lengend Snippet: Detection of antibodies in BAL samples and nasopharyngeal swabs. (a) ELISA curves from plasma, NP swab and BAL for total Ig (heavy and light chain), IgG, IgA and IgM. Measured optical density at 490 nm and a fitted sigmoidal 4 parameter logistic curves are shown. Each curve represents a different subject. The dotted horizontal line represents the cut‐off value for endpoint titre calculations (3× background signal). (b) Interpolated concentrations of IgG, IgA and IgM for paired plasma, NP swab and BAL samples. (c) Ratios of IgG and IgA concentrations in paired samples. Samples from control subjects were used for this figure ( n = 8). Statistical significance was determined by a Friedman's test with Dunn's correction for multiple comparisons.
Article Snippet: Concentrations were interpolated from a standard curve using a
Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Control